Genorama coated microarray slides

Genorama® high quality glass microarray slides are developed and tested primarily for DNA-based applications, however, nucleic acids, proteins, small molecules, cells and extractscan also be immobilized. The activated glass slides are produced under rigorous quality control and tested for low background and good immobilization characteristics. As a result, the slides have a very clean and uniform surface, highly suitable for printing top quality microarrays.

  • Ready-to-print
  • High binding capacity
  • Double-sided uniform coating
  • High signal to noise ratio
  • Low autofluorescence
  • Short DNA immobilization time for SAL
  • Triple quality control
  • Correspond to ISO 9001:2000 standard
  • Boxes of 25 sealed in alufoil

 

Two surface coatings are available


Type

SAL

SA

Coating

aminosilane with linker

aminosilane

For binding

short aminated DNA

unmodified DNA, long aminated DNA

Molecule length

8-75-mer oligonucleotides

Up to 1 kb

Type of binding

Covalent bond

Non-covalent bond

Buffer

 Genorama® Spotting Solution ITM

Genorama® Spotting Solution IITM

The slides produced in two formats: standard size slides 25 x 75 x 1 mm and thin slides 24 x 60 x 0.15 mm (also known as coverslips).


 Six different slide types are available


Code

Coating

W x L x H mm

CAT #

For  binding aminated DNA

 

SAL -1

3-Aminopropyltrimethoxysilane+ 1,4-Phenylenediisothiocyanate

25x75x1.0

G30111

SAL -1 ULTRA*

3-Aminopropyltrimethoxysilane+ 1,4-Phenylenediisothiocyanate

25x75x1.0

G30131

SAL -0.1**

3-Aminopropyltrimethoxysilane+ 1,4-Phenylenediisothiocyanate

24x60x0.15

G30121

For binding unmodified DNA


SA -1

3-Aminopropyltrimethoxysilane

25x75x1.0

G30011

SA - 1 ULTRA***

 3-Aminopropyltrimethoxysilane

25x75x1.0

G30031

SA -0.1

3-Aminopropyltrimethoxysilane

24x60x0.15

G30021

* - same chemistry as the SAL, only the slide substrate is of higher quality level with optically flat surface.
** - 24 x 60 mm slides have one specially refined edge to ensure maximum uniformity of light distribution via total internal reflection excitation in the Genorama® QuattroImagerTM.

*** - same chemistry as the SA, only the slide substrate is of higher quality level with optically flat surface.


SAL advantages compared to epoxy slides:

1.    SAL slides have at least 2 times better binding capacity of aminated DNA than epoxy coated slides.
2.    As SAL slides are more reactive, the probe immobilization time to SAL is much shorter; (only 2 hours for SAL coating, 24 hours for epoxy slides)
3.    Lower pH of the printing solution. (epoxy – pH over 11.0 required to activate the surface. SAL – pH 9.0)
4.    Due to the linker molecule binding occurs further from the surface, theoretically leaving more space for target hybridization.


Additional information

Slides are packed in boxes of 25. Slides sealed in an alufoil bag to protect the activated surface from light and maintain a stable storage environment. Printed slides can be stored at 4°C for up to 6 months. Genorama also provides bar-coded slides upon request.  

Genorama strongly recommends the use of Genorama®Spotting Solutions together with the SAL and SA slides in order to ensure proper quality.