Genorama® QuattroImager™

Genorama® QuattroImagerTM is a four-channel microarray imaging and analysis system, specifically designed for repetitive analysis of multiple slides. International patent application has been filed for Genorama® QuattroImagerTM microarray imaging system.

  • Light field from excited fluorophores will be captured by charge-coupled device camer
  • Genorama® QuattroImagerTM is based on automatic total internal reflection fluorescence system
  • Time required for capturing image for one fluorophore is from 10 to 60 seconds
  • Four lasers are used to excite the fluorescent labels on the APEX microarray


 


The working principle of the Genorama® QuattroImagerTM system is based on total internal reflection fluorescence excitation mechanism combined with a charge coupled device (CCD) detector. The signals from the spectrally separated dyes are resolved with dual selection by laser excitation and band-pass filtering of the emitted fluorescence. 

Four lasers of which light-emitting wavelengths of 473, 532, 594, and 635 nm, are used to excite the fluorescent labels on the APEX microarrays. The optical system consists of light reflectors and cylindrical lenses that transform a beam from each laser into a homogeneously illuminated stripe that is directed into the interior of the glass slide. Due to TIRF of the slide surface, the incoming beam spreads only inside the slide and therefore the intensity of light remains uniform along the entire length of the substrate.


         

Slide excitation mechanism.

The evanescent light field excites the bound fluorophores residing near the surface of the slide, and a gated charge-coupled device (CCD) camera cooled to –10°C records the emitted fluorescence. The respective narrow-band interference filters fixed on the revolving wheel in front of the CCD camera depress noise radiation from scattered laser light on the slide surface. Using one, two, or four dye markers, four images corresponding to four laser wavelengths will be obtained, one for each dye labeled ddNTP

 


Total internal reflection fluorescence excitation with green, yellow, red and blue laser. 


Custom software controls all the parameters of the detection procedure including switching on and off the shutters of the lasers, choosing respective interference filters and setting the recording time at each wavelength. Because of the precise spectral separation of exciting and emitting wavelengths, the system demonstrates a high signal-to-noise ratio. The time required for one fluorescence channel is from 10 to 60 seconds.


Components of Genorama® QuattroImagerTM

  1. Cooled Digital CCD camera system with 2184 x 1472 pixels (pixel size 6.8 x 6.8 μm)
  2. Six position motorized filter wheel
  3. Camera lens
  4. Optical tube
  5. Slide chamber and laser beam inlet
  6. Lens for focusing the laser beam
  7. Common light path with shutters and reflector mirrors

    For the complete set of specifications please ask your representative.


Technical Specifications


Microarray substrate 24 x 60 mm, coverslip-size with one edge specially refined to ensure uniform TIRF excitation
Imaging field
22 x 33 mm
Resolution 15 µm
Sensitivity 2- 5 molecules fluor / µm2
Dynamic range 16 bit
Excitation Wavelengths
4 lasers
  635 nm
  594 nm
  532 nm
  473 nm
Emission Wavelengths 6 position filter wheel with narrow band pass filters
  670 nm position  1
  620 nm position  2
  570 nm position  4
  520 nm position  5
  (custom instrument assembly for other wavelengths available upon request)
Sensor KAF-3200ME CCD
Pixel array
2184 x 1472
Noise reduction Active, Peltier cooling
Sensor operating temperature -10° C
Communication
USB2 port
Dimensions (W x D x H) 55 x 46 x 22 cm (22 x 19 x 9 inches)
Weight 32 kg (72 lbs)
Electrical 100…240 VAC, 50…60Hz