Genorama® QuattroImager™
 Patented Genorama® QuattroImagerTM is a four-channel
microarray imaging and analysis system, specifically designed for repetitive
analysis of multiple slides.
- Light field from excited
fluorophores will be captured by charge-coupled device camera
- Time required for capturing image
for one fluorophore is from 10 to 60 seconds
- Genorama® QuattroImagerTM is based
on automatic total internal reflection fluorescence system
- Four lasers are used to excite
the fluorescent labels on the APEX microarray
The working principle of the Genorama® QuattroImagerTM system is based on total internal reflection fluorescence excitation mechanism combined with a charge coupled device (CCD) detector. The signals from the spectrally separated dyes are resolved with dual selection by laser excitation and band-pass filtering of the emitted fluorescence.
Four lasers of which light-emitting wavelengths of 473, 532, 594, and 635 nm, are used to excite the fluorescent labels on the APEX microarrays. The optical system consists of light reflectors and cylindrical lenses that transform a beam from each laser into a homogeneously illuminated stripe that is directed into the interior of the glass slide. Due to TIRF of the slide surface, the incoming beam spreads only inside the slide and therefore the intensity of light remains uniform along the entire length of the substrate.
Slide excitation mechanism.
The evanescent light field
excites the bound fluorophores residing near the surface of the slide, and a gated
charge-coupled device (CCD) camera cooled to –10°C records the emitted
fluorescence. The respective narrow-band interference filters fixed on the
revolving wheel in front of the CCD camera depress noise radiation from
scattered laser light on the slide surface. Using one, two, or four dye
markers, four images corresponding to four laser wavelengths will be obtained, one
for each dye labeled ddNTP
.JPG)
Total internal reflection fluorescence excitation with green, yellow, red and blue laser.
Custom software controls all the
parameters of the detection procedure including switching on and off the shutters
of the lasers, choosing respective interference filters and setting the
recording time at each wavelength. Because of the precise spectral separation of
exciting and emitting wavelengths, the system demonstrates a high
signal-to-noise ratio. The time required for one fluorescence channel is from 10 to
60 seconds.
 Components of Genorama® QuattroImagerTM
1. Cooled Digital CCD camera system with 2184 x 1472 pixels (pixel size 6.8 x 6.8 ?m)
2. Six position motorized filter wheel
3. Camera lens
4. Optical tube
5. Slide chamber and laser beam inlet
6. Lens for focusing the laser beam
7. Common light path with shutters and reflector mirrors
Technical Specifications
| Microarray substrate |
24 x 60 mm, coverslip-size with one edge specially refined to ensure uniform TIRF excitation
|
Imaging field
|
22 x 33 mm
|
| Resolution |
15 µm |
| Sensitivity |
2- 5 molecules fluor / µm2 |
| Dynamic range |
16 bit |
Excitation Wavelengths
|
4 lasers |
| |
635 nm
|
| |
594 nm
|
| |
532 nm
|
| |
473 nm
|
| Emission Wavelengths |
6 position filter wheel with narrow band pass filters |
| |
670 nm position 1 |
| |
620 nm position 2 |
| |
570 nm position 4 |
| |
520 nm position 5 |
| |
(custom instrument assembly for other wavelengths available upon request)
|
| Sensor |
KAF-3200ME CCD |
Pixel array
|
2184 x 1472 |
| Noise reduction |
Active, Peltier cooling |
| Sensor operating temperature |
-10° C |
Communication
|
USB2 port |
| Dimensions (W x D x H) |
55 x 46 x 22 cm (22 x 19 x 9 inches) |
| Weight |
32 kg (72 lbs) |
| Electrical |
100…240 VAC, 50…60Hz |
|
